yah...
let me say that it is getting hard time for me ,because i am suppose to carry out a PCR for Trp1 gene (from baker´s yeast) and i have not been successful since last 4 weeks.
i almost made every trial and erorr methods.but....
i dont know ,maybe i design the wrong primer.
The question is ,if i already changed all the variables (solution concentrations and annealing temp,.....), doesn't that mean i have somethong wrong with my primers?
The more i search i get results which serve me up more twaddle. Frankly speaking , that's ludicrous.
But at least i enhanced my learning experience ....
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